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Open-loop control in both ATM and Internet service models relies on the leaky bucket to describe traf c ows Despite this apparent convergence, there remain serious doubts about the ef cacy of this choice For present purposes, we consider a leaky bucket as a reservoir of capacity s emptying at rate r and lling due to the controlled input ow Traf c conforms to the leaky bucket descriptor if the reservoir does not over ow and then satis es the inequality A t rt s introduced above The leaky bucket has been chosen mainly because it simpli es the problem of controlling input conformity Its ef cacy depends additionally on being able to choose appropriate parameter values for a given ow and then being able to ef ciently guarantee quality of service by means of admission control.

; Cook, S H; Grif n, D E; Rice, C Roles of nonstructural protein nsP2 and a/b interferons in determining the outcome of Sindbis virus infection J Virol 2002, 76(22), 11254 11264 101 Frolov, I Persistent infection and suppression of host response by alphaviruses Arch Virol Suppl 2004, 18, 139 147 102 White, L J; Wang, J G; Davis, N L; Johnston, R E Role of a/b interferon in Venezuelan equine encephalitis virus pathogenesis: effect of an attenuating mutation in the 50 untranslated region J Virol 2001, 75(8), 3706 3718 103 Thomas, D; Blakqori, G; Wagner, V; Banholzer, M; Kessler, N; Elliott, R M; Haller, O; Weber, F Inhibition of RNA polymerase II phosphorylation by a viral interferon antagonist J Biol Chem 2004, 279, 31471 31477 104 Weber, F; Dunn, E F; Bridgen, A.

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The leaky bucket may be viewed either as a statistical descriptor approximating (or more exactly, providing usefully tight upper bounds on) the actual mean rate and burstiness of a given ow or as the de nition of an envelope into which the traf c must be made to t by shaping Broadly speaking, the rst viewpoint is appropriate for stream traf c, for which excessive shaping delay would be unacceptable, while the second would apply in the case of (aggregates of) elastic traf c Stream traf c should pass transparently through the policer without shaping by choosing large enough bucket rate and capacity parameters Experience with video traces shows that it is very dif cult to de ne a happy medium solution between a leak rate r close to the mean with an excessively large capacity s, and a leak rate close to the peak with a moderate capacity [25].

Acute Oral Testing. Traditionally acute oral toxicity testing has focused on determining the dose that kills half of the animals (i.e., the median lethal dose or LD50), the timing of lethality following acute chemical exposure, as well as observing the onset, nature, severity, and reversibility of toxicity. The LD50 concept was developed by Trevan in 1927. Original testing methods were designed to characterize the dose-response curve by using several animals (usually at least 5/sex) at each of several test doses. Data from a minimum of three doses is required. The LD50 values are presented as estimated doses (mg/kg) with con dence limits. The simplest method for the determination of the LD50 is a graphic one and is based on the assumption that the effect is a

In the former case, although the overall mean rate is accurately predicted, it is hardly a useful traf c characteristic since the rate averaged over periods of several seconds can be signi cantly different In the latter, the rate information is insuf cient to allow signi cant statistical multiplexing gains For elastic ows it is, by de nition, possible to shape traf c to conform to the parameters of a leaky bucket However, it remains dif cult to choose appropriate leaky bucket parameters If the traf c is long-range dependent, as in the case of an aggregation of ows, the performance models studied in this book indicate that queueing behavior is particularly severe.

quantal one (all or none), that the percentage responding in an experimental group is dose related, and that the cumulative effect follows a normal distribution. Data from a typical example (see Figure 11.3), their analysis, and implications are discussed in 11 on acute toxicity. As a result of much recent controversy, the LD50 test has been the subject of considerable regulatory attention and as a result changes in requirements have been promulgated. These changes are intended to obtain more information but, at the same time, use fewer animals.

; Elliott, R M The Bunyamwera virus nonstructural protein NSs inhibits viral RNA synthesis in a minireplicon system Virology 2001, 281(1), 67 74..

For any choice of leak rate r less than the peak rate and a bucket capacity s that is not impractically large, the majority of traf c will be smoothed and admitted to the network at rate r The added value of a nonzero bucket capacity is thus extremely limited for such traf c We conclude that, for both stream and elastic traf c, the leaky bucket constitutes an extremely inadequate descriptor of traf c variability..

Criticism of the LD50 Test. The criticisms of the test include:

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